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a16789 03  (Thermo Fisher)


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    Thermo Fisher a16789 03
    A16789 03, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a16789+03/L+FUCOSE+98/pm40992372-229-60-74
    Average 94 stars, based on 1 article reviews
    a16789 03 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: Hinton; 75 N/A TOP10 cells Thermo Fisher Cat #C404010 BL21 cells Thermo Fisher Cat #C600003 Biological samples Porcine Intestines Research 87, Boylston, MA 01505 N/A Porcine Stomachs LeMay and Sons Beef Packaging, Goffstown, NH 03045 N/A Chemicals, peptides, and recombinant proteins LB Broth, Miller (Luria Bertani) BD Difco Cat# 244620 Agar Sigma Cat# A1296 M9 AMRESCO Cat# J863-500G Dextrose Sigma Cat# G6152-1KG Lot# SLCB6282 NaCl Sigma Cat# S9888-5KG MgSO 4 Sigma Cat# M2773-500G CaCl Sigma Cat# C5670-100G Chloramphenicol MPBio Cat# 190321 Kanamycin Sigma Cat# K1377 Carbenicillin Sigma Cat# C3416-250MG Nalidixic acid Sigma Cat# N4382 Dulbecco’s Minimum Essential Media (DMEM) Gibco Cat# 11965 Fetal Bovine Serum (FBS) Gibco Cat# 10500064 DPBS Gibco Cat# 14040133 Gentamicin Sigma Cat# G1264 Triton X-100 Sigma Cat# T9284-500ML NaN 3 Sigma Cat# S2002-100G NH 4 OH, Solution 30–33% NH 3 in H 2 O Sigma Cat# 05002-1L (Continued on next page) 16 Cell Reports ▪▪, 116304, ▪▪, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER (NH 4 ) 2 CO 3 Sigma Cat# 207861-500G Boric Acid Sigma Cat# B6768 Benzamidine HCl Sigma Cat# 434760-25G EDTA Sigma Cat# EDS-500G 2,3 ′ -Dibromoacetophenone Sigma Cat# 654787-5G Phenylmethylsulphonyl Fluoride Sigma Cat# 78830-5G D-Galactose Sigma Cat# G0625-100G Lot# 060M00631V N-acetyl-D-galactosamine Sigma Cat# A2795-500MG Lot# BCCD6801 N-acetyl-D-glucosamine Fluka Cat# WA20549 Lot# 1323294 L-Fucose Sigma Cat# A16789.03 Lot# R08J007 N-Acetylneuraminic acid Sigma Cat# A0812-100MG Lot# BCCD5508 LB broth (glycopolypeptide synthesis) Invitrogen Cat# 12780-052 Terrific broth (glycopolypeptide synthesis) Invitrogen Cat# 22711-022 LB agar (glycopolypeptide synthesis) Invitrogen Cat# 22700-025 Carbenicillin (glycopolypeptide synthesis) Thermo Fisher Cat# J61949-06 Imidazole (glycopolypeptide synthesis) VWR Cat# 0527-100G Tris (glycopolypeptide synthesis) VWR Cat# 0497-500G NaCl (glycopolypeptide synthesis) Fisher Cat# S271-3 Glycerol (glycopolypeptide synthesis) Fisher Cat# BP229-4 CTP (glycopolypeptide synthesis) Alfa Aesar Cat# J62238 Sodium pyruvate (glycopolypeptide synthesis) VWR Cat# 0342-100G ManNAc (glycopolypeptide synthesis) Alfa Aesar Cat# L11167 MgCl 2 (glycopolypeptide synthesis) Fisher Cat# BP214-500 Critical commercial assays MasterPure Complete DNA and RNA Purification Kit Biosearch Technologies Cat# MC85200 Lot# 34146 ProtoScript II First Strand cDNA Synthesis kit NEB Cat# E6560L Turbo DNA-Free Kit Invitrogen Cat# AM1907 SYBR PowerUp Master Mix Applied Biosystems Cat# A25778 NEBNext rRNA Depletion Kit NEB Cat# E7850X LIVE/DEAD Cell Imaging Kit Invitrogen Cat# R37601 B-PER Reagent Thermo scientific Cat# 78243 Halt Protease Inhibitor Cocktail (100X) Invitrogen Cat# 87786 Pierce BCA Protein Assay Kit Thermo scientific Cat# 23227 Deposited data RNA-seq This study GEO: GSE278090 Underlying numerical data for main figures This study Dryad: https://doi.org/10.5061/dryad. ..

    cDNA Synthesis:

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: Hinton; 75 N/A TOP10 cells Thermo Fisher Cat #C404010 BL21 cells Thermo Fisher Cat #C600003 Biological samples Porcine Intestines Research 87, Boylston, MA 01505 N/A Porcine Stomachs LeMay and Sons Beef Packaging, Goffstown, NH 03045 N/A Chemicals, peptides, and recombinant proteins LB Broth, Miller (Luria Bertani) BD Difco Cat# 244620 Agar Sigma Cat# A1296 M9 AMRESCO Cat# J863-500G Dextrose Sigma Cat# G6152-1KG Lot# SLCB6282 NaCl Sigma Cat# S9888-5KG MgSO 4 Sigma Cat# M2773-500G CaCl Sigma Cat# C5670-100G Chloramphenicol MPBio Cat# 190321 Kanamycin Sigma Cat# K1377 Carbenicillin Sigma Cat# C3416-250MG Nalidixic acid Sigma Cat# N4382 Dulbecco’s Minimum Essential Media (DMEM) Gibco Cat# 11965 Fetal Bovine Serum (FBS) Gibco Cat# 10500064 DPBS Gibco Cat# 14040133 Gentamicin Sigma Cat# G1264 Triton X-100 Sigma Cat# T9284-500ML NaN 3 Sigma Cat# S2002-100G NH 4 OH, Solution 30–33% NH 3 in H 2 O Sigma Cat# 05002-1L (Continued on next page) 16 Cell Reports ▪▪, 116304, ▪▪, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER (NH 4 ) 2 CO 3 Sigma Cat# 207861-500G Boric Acid Sigma Cat# B6768 Benzamidine HCl Sigma Cat# 434760-25G EDTA Sigma Cat# EDS-500G 2,3 ′ -Dibromoacetophenone Sigma Cat# 654787-5G Phenylmethylsulphonyl Fluoride Sigma Cat# 78830-5G D-Galactose Sigma Cat# G0625-100G Lot# 060M00631V N-acetyl-D-galactosamine Sigma Cat# A2795-500MG Lot# BCCD6801 N-acetyl-D-glucosamine Fluka Cat# WA20549 Lot# 1323294 L-Fucose Sigma Cat# A16789.03 Lot# R08J007 N-Acetylneuraminic acid Sigma Cat# A0812-100MG Lot# BCCD5508 LB broth (glycopolypeptide synthesis) Invitrogen Cat# 12780-052 Terrific broth (glycopolypeptide synthesis) Invitrogen Cat# 22711-022 LB agar (glycopolypeptide synthesis) Invitrogen Cat# 22700-025 Carbenicillin (glycopolypeptide synthesis) Thermo Fisher Cat# J61949-06 Imidazole (glycopolypeptide synthesis) VWR Cat# 0527-100G Tris (glycopolypeptide synthesis) VWR Cat# 0497-500G NaCl (glycopolypeptide synthesis) Fisher Cat# S271-3 Glycerol (glycopolypeptide synthesis) Fisher Cat# BP229-4 CTP (glycopolypeptide synthesis) Alfa Aesar Cat# J62238 Sodium pyruvate (glycopolypeptide synthesis) VWR Cat# 0342-100G ManNAc (glycopolypeptide synthesis) Alfa Aesar Cat# L11167 MgCl 2 (glycopolypeptide synthesis) Fisher Cat# BP214-500 Critical commercial assays MasterPure Complete DNA and RNA Purification Kit Biosearch Technologies Cat# MC85200 Lot# 34146 ProtoScript II First Strand cDNA Synthesis kit NEB Cat# E6560L Turbo DNA-Free Kit Invitrogen Cat# AM1907 SYBR PowerUp Master Mix Applied Biosystems Cat# A25778 NEBNext rRNA Depletion Kit NEB Cat# E7850X LIVE/DEAD Cell Imaging Kit Invitrogen Cat# R37601 B-PER Reagent Thermo scientific Cat# 78243 Halt Protease Inhibitor Cocktail (100X) Invitrogen Cat# 87786 Pierce BCA Protein Assay Kit Thermo scientific Cat# 23227 Deposited data RNA-seq This study GEO: GSE278090 Underlying numerical data for main figures This study Dryad: https://doi.org/10.5061/dryad. ..

    Imaging:

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: Hinton; 75 N/A TOP10 cells Thermo Fisher Cat #C404010 BL21 cells Thermo Fisher Cat #C600003 Biological samples Porcine Intestines Research 87, Boylston, MA 01505 N/A Porcine Stomachs LeMay and Sons Beef Packaging, Goffstown, NH 03045 N/A Chemicals, peptides, and recombinant proteins LB Broth, Miller (Luria Bertani) BD Difco Cat# 244620 Agar Sigma Cat# A1296 M9 AMRESCO Cat# J863-500G Dextrose Sigma Cat# G6152-1KG Lot# SLCB6282 NaCl Sigma Cat# S9888-5KG MgSO 4 Sigma Cat# M2773-500G CaCl Sigma Cat# C5670-100G Chloramphenicol MPBio Cat# 190321 Kanamycin Sigma Cat# K1377 Carbenicillin Sigma Cat# C3416-250MG Nalidixic acid Sigma Cat# N4382 Dulbecco’s Minimum Essential Media (DMEM) Gibco Cat# 11965 Fetal Bovine Serum (FBS) Gibco Cat# 10500064 DPBS Gibco Cat# 14040133 Gentamicin Sigma Cat# G1264 Triton X-100 Sigma Cat# T9284-500ML NaN 3 Sigma Cat# S2002-100G NH 4 OH, Solution 30–33% NH 3 in H 2 O Sigma Cat# 05002-1L (Continued on next page) 16 Cell Reports ▪▪, 116304, ▪▪, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER (NH 4 ) 2 CO 3 Sigma Cat# 207861-500G Boric Acid Sigma Cat# B6768 Benzamidine HCl Sigma Cat# 434760-25G EDTA Sigma Cat# EDS-500G 2,3 ′ -Dibromoacetophenone Sigma Cat# 654787-5G Phenylmethylsulphonyl Fluoride Sigma Cat# 78830-5G D-Galactose Sigma Cat# G0625-100G Lot# 060M00631V N-acetyl-D-galactosamine Sigma Cat# A2795-500MG Lot# BCCD6801 N-acetyl-D-glucosamine Fluka Cat# WA20549 Lot# 1323294 L-Fucose Sigma Cat# A16789.03 Lot# R08J007 N-Acetylneuraminic acid Sigma Cat# A0812-100MG Lot# BCCD5508 LB broth (glycopolypeptide synthesis) Invitrogen Cat# 12780-052 Terrific broth (glycopolypeptide synthesis) Invitrogen Cat# 22711-022 LB agar (glycopolypeptide synthesis) Invitrogen Cat# 22700-025 Carbenicillin (glycopolypeptide synthesis) Thermo Fisher Cat# J61949-06 Imidazole (glycopolypeptide synthesis) VWR Cat# 0527-100G Tris (glycopolypeptide synthesis) VWR Cat# 0497-500G NaCl (glycopolypeptide synthesis) Fisher Cat# S271-3 Glycerol (glycopolypeptide synthesis) Fisher Cat# BP229-4 CTP (glycopolypeptide synthesis) Alfa Aesar Cat# J62238 Sodium pyruvate (glycopolypeptide synthesis) VWR Cat# 0342-100G ManNAc (glycopolypeptide synthesis) Alfa Aesar Cat# L11167 MgCl 2 (glycopolypeptide synthesis) Fisher Cat# BP214-500 Critical commercial assays MasterPure Complete DNA and RNA Purification Kit Biosearch Technologies Cat# MC85200 Lot# 34146 ProtoScript II First Strand cDNA Synthesis kit NEB Cat# E6560L Turbo DNA-Free Kit Invitrogen Cat# AM1907 SYBR PowerUp Master Mix Applied Biosystems Cat# A25778 NEBNext rRNA Depletion Kit NEB Cat# E7850X LIVE/DEAD Cell Imaging Kit Invitrogen Cat# R37601 B-PER Reagent Thermo scientific Cat# 78243 Halt Protease Inhibitor Cocktail (100X) Invitrogen Cat# 87786 Pierce BCA Protein Assay Kit Thermo scientific Cat# 23227 Deposited data RNA-seq This study GEO: GSE278090 Underlying numerical data for main figures This study Dryad: https://doi.org/10.5061/dryad. ..

    Protease Inhibitor:

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: Hinton; 75 N/A TOP10 cells Thermo Fisher Cat #C404010 BL21 cells Thermo Fisher Cat #C600003 Biological samples Porcine Intestines Research 87, Boylston, MA 01505 N/A Porcine Stomachs LeMay and Sons Beef Packaging, Goffstown, NH 03045 N/A Chemicals, peptides, and recombinant proteins LB Broth, Miller (Luria Bertani) BD Difco Cat# 244620 Agar Sigma Cat# A1296 M9 AMRESCO Cat# J863-500G Dextrose Sigma Cat# G6152-1KG Lot# SLCB6282 NaCl Sigma Cat# S9888-5KG MgSO 4 Sigma Cat# M2773-500G CaCl Sigma Cat# C5670-100G Chloramphenicol MPBio Cat# 190321 Kanamycin Sigma Cat# K1377 Carbenicillin Sigma Cat# C3416-250MG Nalidixic acid Sigma Cat# N4382 Dulbecco’s Minimum Essential Media (DMEM) Gibco Cat# 11965 Fetal Bovine Serum (FBS) Gibco Cat# 10500064 DPBS Gibco Cat# 14040133 Gentamicin Sigma Cat# G1264 Triton X-100 Sigma Cat# T9284-500ML NaN 3 Sigma Cat# S2002-100G NH 4 OH, Solution 30–33% NH 3 in H 2 O Sigma Cat# 05002-1L (Continued on next page) 16 Cell Reports ▪▪, 116304, ▪▪, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER (NH 4 ) 2 CO 3 Sigma Cat# 207861-500G Boric Acid Sigma Cat# B6768 Benzamidine HCl Sigma Cat# 434760-25G EDTA Sigma Cat# EDS-500G 2,3 ′ -Dibromoacetophenone Sigma Cat# 654787-5G Phenylmethylsulphonyl Fluoride Sigma Cat# 78830-5G D-Galactose Sigma Cat# G0625-100G Lot# 060M00631V N-acetyl-D-galactosamine Sigma Cat# A2795-500MG Lot# BCCD6801 N-acetyl-D-glucosamine Fluka Cat# WA20549 Lot# 1323294 L-Fucose Sigma Cat# A16789.03 Lot# R08J007 N-Acetylneuraminic acid Sigma Cat# A0812-100MG Lot# BCCD5508 LB broth (glycopolypeptide synthesis) Invitrogen Cat# 12780-052 Terrific broth (glycopolypeptide synthesis) Invitrogen Cat# 22711-022 LB agar (glycopolypeptide synthesis) Invitrogen Cat# 22700-025 Carbenicillin (glycopolypeptide synthesis) Thermo Fisher Cat# J61949-06 Imidazole (glycopolypeptide synthesis) VWR Cat# 0527-100G Tris (glycopolypeptide synthesis) VWR Cat# 0497-500G NaCl (glycopolypeptide synthesis) Fisher Cat# S271-3 Glycerol (glycopolypeptide synthesis) Fisher Cat# BP229-4 CTP (glycopolypeptide synthesis) Alfa Aesar Cat# J62238 Sodium pyruvate (glycopolypeptide synthesis) VWR Cat# 0342-100G ManNAc (glycopolypeptide synthesis) Alfa Aesar Cat# L11167 MgCl 2 (glycopolypeptide synthesis) Fisher Cat# BP214-500 Critical commercial assays MasterPure Complete DNA and RNA Purification Kit Biosearch Technologies Cat# MC85200 Lot# 34146 ProtoScript II First Strand cDNA Synthesis kit NEB Cat# E6560L Turbo DNA-Free Kit Invitrogen Cat# AM1907 SYBR PowerUp Master Mix Applied Biosystems Cat# A25778 NEBNext rRNA Depletion Kit NEB Cat# E7850X LIVE/DEAD Cell Imaging Kit Invitrogen Cat# R37601 B-PER Reagent Thermo scientific Cat# 78243 Halt Protease Inhibitor Cocktail (100X) Invitrogen Cat# 87786 Pierce BCA Protein Assay Kit Thermo scientific Cat# 23227 Deposited data RNA-seq This study GEO: GSE278090 Underlying numerical data for main figures This study Dryad: https://doi.org/10.5061/dryad. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: Hinton; 75 N/A TOP10 cells Thermo Fisher Cat #C404010 BL21 cells Thermo Fisher Cat #C600003 Biological samples Porcine Intestines Research 87, Boylston, MA 01505 N/A Porcine Stomachs LeMay and Sons Beef Packaging, Goffstown, NH 03045 N/A Chemicals, peptides, and recombinant proteins LB Broth, Miller (Luria Bertani) BD Difco Cat# 244620 Agar Sigma Cat# A1296 M9 AMRESCO Cat# J863-500G Dextrose Sigma Cat# G6152-1KG Lot# SLCB6282 NaCl Sigma Cat# S9888-5KG MgSO 4 Sigma Cat# M2773-500G CaCl Sigma Cat# C5670-100G Chloramphenicol MPBio Cat# 190321 Kanamycin Sigma Cat# K1377 Carbenicillin Sigma Cat# C3416-250MG Nalidixic acid Sigma Cat# N4382 Dulbecco’s Minimum Essential Media (DMEM) Gibco Cat# 11965 Fetal Bovine Serum (FBS) Gibco Cat# 10500064 DPBS Gibco Cat# 14040133 Gentamicin Sigma Cat# G1264 Triton X-100 Sigma Cat# T9284-500ML NaN 3 Sigma Cat# S2002-100G NH 4 OH, Solution 30–33% NH 3 in H 2 O Sigma Cat# 05002-1L (Continued on next page) 16 Cell Reports ▪▪, 116304, ▪▪, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER (NH 4 ) 2 CO 3 Sigma Cat# 207861-500G Boric Acid Sigma Cat# B6768 Benzamidine HCl Sigma Cat# 434760-25G EDTA Sigma Cat# EDS-500G 2,3 ′ -Dibromoacetophenone Sigma Cat# 654787-5G Phenylmethylsulphonyl Fluoride Sigma Cat# 78830-5G D-Galactose Sigma Cat# G0625-100G Lot# 060M00631V N-acetyl-D-galactosamine Sigma Cat# A2795-500MG Lot# BCCD6801 N-acetyl-D-glucosamine Fluka Cat# WA20549 Lot# 1323294 L-Fucose Sigma Cat# A16789.03 Lot# R08J007 N-Acetylneuraminic acid Sigma Cat# A0812-100MG Lot# BCCD5508 LB broth (glycopolypeptide synthesis) Invitrogen Cat# 12780-052 Terrific broth (glycopolypeptide synthesis) Invitrogen Cat# 22711-022 LB agar (glycopolypeptide synthesis) Invitrogen Cat# 22700-025 Carbenicillin (glycopolypeptide synthesis) Thermo Fisher Cat# J61949-06 Imidazole (glycopolypeptide synthesis) VWR Cat# 0527-100G Tris (glycopolypeptide synthesis) VWR Cat# 0497-500G NaCl (glycopolypeptide synthesis) Fisher Cat# S271-3 Glycerol (glycopolypeptide synthesis) Fisher Cat# BP229-4 CTP (glycopolypeptide synthesis) Alfa Aesar Cat# J62238 Sodium pyruvate (glycopolypeptide synthesis) VWR Cat# 0342-100G ManNAc (glycopolypeptide synthesis) Alfa Aesar Cat# L11167 MgCl 2 (glycopolypeptide synthesis) Fisher Cat# BP214-500 Critical commercial assays MasterPure Complete DNA and RNA Purification Kit Biosearch Technologies Cat# MC85200 Lot# 34146 ProtoScript II First Strand cDNA Synthesis kit NEB Cat# E6560L Turbo DNA-Free Kit Invitrogen Cat# AM1907 SYBR PowerUp Master Mix Applied Biosystems Cat# A25778 NEBNext rRNA Depletion Kit NEB Cat# E7850X LIVE/DEAD Cell Imaging Kit Invitrogen Cat# R37601 B-PER Reagent Thermo scientific Cat# 78243 Halt Protease Inhibitor Cocktail (100X) Invitrogen Cat# 87786 Pierce BCA Protein Assay Kit Thermo scientific Cat# 23227 Deposited data RNA-seq This study GEO: GSE278090 Underlying numerical data for main figures This study Dryad: https://doi.org/10.5061/dryad. ..

    RNA Sequencing:

    Article Title: Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.
    Article Snippet: Hinton; 75 N/A TOP10 cells Thermo Fisher Cat #C404010 BL21 cells Thermo Fisher Cat #C600003 Biological samples Porcine Intestines Research 87, Boylston, MA 01505 N/A Porcine Stomachs LeMay and Sons Beef Packaging, Goffstown, NH 03045 N/A Chemicals, peptides, and recombinant proteins LB Broth, Miller (Luria Bertani) BD Difco Cat# 244620 Agar Sigma Cat# A1296 M9 AMRESCO Cat# J863-500G Dextrose Sigma Cat# G6152-1KG Lot# SLCB6282 NaCl Sigma Cat# S9888-5KG MgSO 4 Sigma Cat# M2773-500G CaCl Sigma Cat# C5670-100G Chloramphenicol MPBio Cat# 190321 Kanamycin Sigma Cat# K1377 Carbenicillin Sigma Cat# C3416-250MG Nalidixic acid Sigma Cat# N4382 Dulbecco’s Minimum Essential Media (DMEM) Gibco Cat# 11965 Fetal Bovine Serum (FBS) Gibco Cat# 10500064 DPBS Gibco Cat# 14040133 Gentamicin Sigma Cat# G1264 Triton X-100 Sigma Cat# T9284-500ML NaN 3 Sigma Cat# S2002-100G NH 4 OH, Solution 30–33% NH 3 in H 2 O Sigma Cat# 05002-1L (Continued on next page) 16 Cell Reports ▪▪, 116304, ▪▪, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER (NH 4 ) 2 CO 3 Sigma Cat# 207861-500G Boric Acid Sigma Cat# B6768 Benzamidine HCl Sigma Cat# 434760-25G EDTA Sigma Cat# EDS-500G 2,3 ′ -Dibromoacetophenone Sigma Cat# 654787-5G Phenylmethylsulphonyl Fluoride Sigma Cat# 78830-5G D-Galactose Sigma Cat# G0625-100G Lot# 060M00631V N-acetyl-D-galactosamine Sigma Cat# A2795-500MG Lot# BCCD6801 N-acetyl-D-glucosamine Fluka Cat# WA20549 Lot# 1323294 L-Fucose Sigma Cat# A16789.03 Lot# R08J007 N-Acetylneuraminic acid Sigma Cat# A0812-100MG Lot# BCCD5508 LB broth (glycopolypeptide synthesis) Invitrogen Cat# 12780-052 Terrific broth (glycopolypeptide synthesis) Invitrogen Cat# 22711-022 LB agar (glycopolypeptide synthesis) Invitrogen Cat# 22700-025 Carbenicillin (glycopolypeptide synthesis) Thermo Fisher Cat# J61949-06 Imidazole (glycopolypeptide synthesis) VWR Cat# 0527-100G Tris (glycopolypeptide synthesis) VWR Cat# 0497-500G NaCl (glycopolypeptide synthesis) Fisher Cat# S271-3 Glycerol (glycopolypeptide synthesis) Fisher Cat# BP229-4 CTP (glycopolypeptide synthesis) Alfa Aesar Cat# J62238 Sodium pyruvate (glycopolypeptide synthesis) VWR Cat# 0342-100G ManNAc (glycopolypeptide synthesis) Alfa Aesar Cat# L11167 MgCl 2 (glycopolypeptide synthesis) Fisher Cat# BP214-500 Critical commercial assays MasterPure Complete DNA and RNA Purification Kit Biosearch Technologies Cat# MC85200 Lot# 34146 ProtoScript II First Strand cDNA Synthesis kit NEB Cat# E6560L Turbo DNA-Free Kit Invitrogen Cat# AM1907 SYBR PowerUp Master Mix Applied Biosystems Cat# A25778 NEBNext rRNA Depletion Kit NEB Cat# E7850X LIVE/DEAD Cell Imaging Kit Invitrogen Cat# R37601 B-PER Reagent Thermo scientific Cat# 78243 Halt Protease Inhibitor Cocktail (100X) Invitrogen Cat# 87786 Pierce BCA Protein Assay Kit Thermo scientific Cat# 23227 Deposited data RNA-seq This study GEO: GSE278090 Underlying numerical data for main figures This study Dryad: https://doi.org/10.5061/dryad. ..



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